Figure 9
From: A novel paper MAP method for rapid high resolution histological analysis
Application of Paper-MAP for tissue clearing and expansion in human brain tumor biopsy. (a) Optical transparency of human brain tumor biopsy achieved via Tissue-MAP. (b) Optical transparency of human brain tumor biopsy achieved via Paper-MAP. (c) Immunostaining for EGFR (green), Lectin (red), c-Mer (green), and Olig2 (green) in human brain tumor biopsy sections processed via Paper-MAP. Images of before (left, fixed in 4% PFA) and after Paper-MAP (right) was taken with the same 10 × and 40 × objective lens and z-stacked (range: 100-μm) for comparison. Scale bars: green = 1-mm, white = 100-μm.
