Figure 3

Representative photomicrographs of annotated necrotic area (defect site) with empty lacunae from no-gel group (a); quantification of necrotic area (b) showed a significant positive effect of the VEGF-gel compared to no-gel and gel-alone groups (****p < 0.0001, ***p < 0.001, **p < 0.01). Blue outlines indicate included areas and green outlines depict excluded areas whilst quantification was performed.