Figure 4

PCBP1 selectively binds to mRNA to regulate alternative splicing. (A) Venn diagram showing the overlap of PCBP1-bound peaks and PCBP1-regulated alternatively NIR splicing events (use NIR region), and the PCBP1-bound peaks is the union of two IP samples, bound peaks cluster (several peaks will appear together, which can be considered as one peak). (B) Bar plot exhibited the most enriched GO biological process results of the overlap peak genes in Fig. (A). (C) Venn diagram showing the overlap of PCBP1-bound peaks and PCBP1-regulated alternatively NIR splicing events (Use Symbol), and the PCBP1-bound peaks is the union of two IP samples. (D) Bar plot exhibited the most enriched GO biological process results of the overlap peak genes in Fig. (C). (E) IGV-sashimi plot showed the alternative 3′ splicing sites events (A3SS) in CELF1. Reads distribution of each alternative splicing event was plotted in the left panel with the transcripts of each gene shown below. The schematic diagrams depict the structures of ASEs. (F) IGV-sashimi plot showed the alternative 5’ splicing sites events (A5SS) in SPHK1. Reads distribution of each alternative splicing event was plotted in the left panel with the transcripts of each gene shown below. The schematic diagrams depict the structures of ASEs. PCBP1, Poly C Binding Protein 1; mRNA, messenger ribonucleic acid; NIR, non-intron-retention regulated; GO, gene ontology. IGV, The Integrative Genomics Viewer.