Figure 2 | Scientific Reports

Figure 2

From: Effect of human secretory calcium-binding phosphoprotein proline-glutamine rich 1 protein on Porphyromonas gingivalis and identification of its active portions

Figure 2

Determination of the working concentration of SCPPPQ1 that affect P. gingivalis growing conditions. Comparison of the difference in growth of the bacteria on a blood agar petri dish in (a) the presence of buffer alone (negative control) or (b–f) various concentrations of + SCPPPQ1. (b–f) Enlargements of the boxed areas in the blood agar plate (left panel). (a) Under control conditions the bacteria formed a relatively uniform layer that appeared black in photographs, (b–f) while in presence of proteins, this layer was interrupted producing a mosaic of black areas containing bacteria (white arrowhead) and white areas of various sizes that indicate absence/paucity of bacteria (white arrow). Qualitative evaluation suggests that the effect of SCPPPQ1 on the bacteria (white areas) started at 5 µM (weak) and plateaued at 20 µM (see Table 1). These results are representative of three experiments.

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