Figure 4 | Scientific Reports

Figure 4

From: Effect of human secretory calcium-binding phosphoprotein proline-glutamine rich 1 protein on Porphyromonas gingivalis and identification of its active portions

Figure 4

Effect of SCPPPQ1 on formation of P. gingivalis bacterial aggregates. (a) Visual inspection of bacterial cultures in test tubes from the negative control (left) and + SCPPPQ1 treated (right) samples at 20 min. A representative image from six different experiments is shown. (b) Super-resolution fluorescence images of bacteria (membrane stained in red with FM 4–64 dye) from the negative control (left) and + SCPPPQ1 treated (right) samples at 20 min. Images are representative from five different experiments. (c) FACS results of the fold change in relative aggregates volume over time normalized on the negative control at 0 min. Data are represented as mean ± standard error of mean (n = 4). Significance was determined by two-tailed Student’s t test analysis (**p < 0.01; ***p < 0.001; ****p < 0.0001).

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