Figure 5

Localisation of SCPPPQ1 on P. gingivalis using super-resolution fluorescence imaging. (a–c) Images of bacteria incubated with the buffer only (negative control) at 20 min. (b,c) Enlargements of corresponding boxed areas in (a). (d–f) Images of bacteria incubated with + SCPPPQ1 at 20 min. (e, f) Enlargements of corresponding boxed areas in (d). (e) Upper dashed box is a magnification of the lower one. Bacterial nucleic acids were labeled with SYTO 9 (green fluorescence) and SCPPPQ1 with Alexa Fluor-546 secondary antibody (red fluorescence). These images are representative from four different experiments.