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Figure 1

From: Quantitative proteomic screen identifies annexin A2 as a host target for Salmonella pathogenicity island-2 effectors SopD2 and PipB2

Figure 1

ssrB overexpression increases T3SS2 secretion, but not T3SS1 secretion. Images shown are representative of n = 3 experiments. (a) Secreted proteins from indicated strains grown under SPI-1 inducing conditions were separated by SDS-PAGE and stained with Coomassie Brilliant R250. (b,c) Secreted proteins from indicated strains grown under SPI-2 inducing conditions were precipitated from sterilized culture supernatant and separated on an SDS-PAGE gel and immunoblotted with α-SseD (b) or α-SseB (c). (a–c) Images were cropped where indicated by dividing lines. Original Coomassie stained SDS-PAGE gel and western blots are shown in Supplemental Fig. S2. (d) Densitometric analysis of SPI-1 secreted effectors SipA, SipC, and SopE as determined by 3 SDS-PAGE gels stained by Coomassie. Ns not significant as determined by a Kruskal–Wallis test with Dunn’s correction for multiple comparisons. (e) Fold change determined by densitometry of 3 Western blots from independent experiments. Mean fold change ± standard deviation is shown. Fold change = (Densitometry of T3SS2+)/(Densitometry of wild type). Dashed line = 1.

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