Figure 6

Annexin A2 contributes to the precise intracellular positioning of the SCV during infection. (a) STRING network showing both direct and indirect protein associations between AnxA2 and previously identified host proteins known to be involved in SIF biogenesis. Line thickness indicates the strength of data supporting the interaction with a minimum confidence of interaction score of 0.4. (b) Annexin A2 expression in Hela cells treated with either control shRNA (left lane) or with Annexin A2 shRNA (right lane) as determined by Western blot from whole cell lysate. Beta Tubulin was used as a loading control. Original Western blots are shown in Supplemental Fig. S5. (c) Distribution of Salmonella in infected HeLa cells treated with either control shRNA or Annexin A2 shRNA. shRNA-treated HeLa cells infected with wild type Salmonella were fixed at 8 h post infection, immunostained for Salmonella (red), LAMP1 (green), and actin (grey), and the nucleus was stained with DAPI (blue). The nearest edge distance from Salmonella to nucleus was measured in infected cells. The mean plus or minus SEM for three experiments is shown (n = 3). At least 200 distances per experiment were measured. The p-value is as indicated as determined by a Mann–Whitney test. (d) Select representative images of wild type Salmonella-infected HeLa cells treated with either control shRNA or Annexin A2 shRNA.