Table 2 Plasmids used in this study.
Plasmid designation | Relevant characteristics/genotype | Source/references |
---|---|---|
pRE112-ssrB | pRE112 containing ssrB and homologous regions for insertion | This study |
pRE112ΔsifA | pRE112 containing homologous regions surrounding sifA for chromosomal sifA deletion | |
pRE112ΔsopD2 | pRE112 containing homologous regions surrounding sopD2 for chromosomal sopD2 deletion | |
pRE112ΔpipB2 | pRE112 containing homologous regions surrounding pipB2 for chromosomal pipB2 deletion | |
pRE112ΔsteA | pRE112 containing homologous regions surrounding steA for chromosomal steA deletion | |
pRE112ΔsseJ | pRE112 containing homologous regions surrounding sseJ for chromosomal sseJ deletion | |
pRE112ΔsseF | pRE112 containing homologous regions surrounding sseF for chromosomal sseF deletion | |
pSifA-2HA | Tandem HA Tag in the middle of SifA in pACYC184 | |
pSopD2-2HA | SopD2 with a tandem C-terminal HA tag in pACYC184 | |
pPipB2-2HA | PipB2 with a tandem C-terminal HA tag in pACYC184 | This study |
pSteA-2HA | SteA with a tandem C-terminal HA tag in pACYC184 | This study |
pSseJ-2HA | SseJ with a tandem C-terminal HA tag in pACYC184 | This study |
pSseF-2HA | SseF with a tandem C-terminal HA tag in pACYC184 | This study |
pACYC-2HA | Tandem HA Tag under the control of the native pipB2 promoter in pACYC184 | This study |