Table 2 Plasmids used in this study.

From: Quantitative proteomic screen identifies annexin A2 as a host target for Salmonella pathogenicity island-2 effectors SopD2 and PipB2

Plasmid designation

Relevant characteristics/genotype

Source/references

pRE112-ssrB

pRE112 containing ssrB and homologous regions for insertion

This study

pRE112ΔsifA

pRE112 containing homologous regions surrounding sifA for chromosomal sifA deletion

11

pRE112ΔsopD2

pRE112 containing homologous regions surrounding sopD2 for chromosomal sopD2 deletion

11

pRE112ΔpipB2

pRE112 containing homologous regions surrounding pipB2 for chromosomal pipB2 deletion

11

pRE112ΔsteA

pRE112 containing homologous regions surrounding steA for chromosomal steA deletion

11

pRE112ΔsseJ

pRE112 containing homologous regions surrounding sseJ for chromosomal sseJ deletion

11

pRE112ΔsseF

pRE112 containing homologous regions surrounding sseF for chromosomal sseF deletion

11

pSifA-2HA

Tandem HA Tag in the middle of SifA in pACYC184

20

pSopD2-2HA

SopD2 with a tandem C-terminal HA tag in pACYC184

21

pPipB2-2HA

PipB2 with a tandem C-terminal HA tag in pACYC184

This study

pSteA-2HA

SteA with a tandem C-terminal HA tag in pACYC184

This study

pSseJ-2HA

SseJ with a tandem C-terminal HA tag in pACYC184

This study

pSseF-2HA

SseF with a tandem C-terminal HA tag in pACYC184

This study

pACYC-2HA

Tandem HA Tag under the control of the native pipB2 promoter in pACYC184

This study