Table 3 Primers used in this study.

From: Quantitative proteomic screen identifies annexin A2 as a host target for Salmonella pathogenicity island-2 effectors SopD2 and PipB2

Primer name

5Ęą to 3Ęą sequence

Description

KK_001

GAGCTCTCCCGGGAATTCATGCAGTTCAC

Linearization of pRE112 maintaining SacI and KpnI cut sites

KK_002

GGTACCTCTAGAAGAAGCTTGGGA

KK_003

CAAGCTTCTTCTAGAGGTACCCCGTCTTCGCTGATATCCCAC

Amplification of region upstream of ssrB insertion

KK_004

CTCAGATAATCAACATATCGAAAGAAATTTTTC

KK_005

CGATATGTTGATTATCTGAGCAGATGATATGGTCATTAATAGCAAG

Amplification of ssrB and native promoter

KK_006

GCGTTAGTGGTATTAATCGTTAATACTCTATTAACCTCATTCTTCGGGC

KK_007

GAGGTTAATAGAGTATTAACGATTAATACCACTAACGCTAAAACGCAC

Amplification of region downstream of ssrB insertion

KK_008

ATGAATTCCCGGGAGAGCTCGGTGATGCGGTAATGTCGCTGC

KK_009

GTACGCGTCGACCCGAGACGGTAGCCTGATTGAGTTAAACG

Amplification of pipB2 plus native promoter, SalI and XhoI cut sites

KK_010

GTACGCCTCGAGAATATTTTCACTATAAAATTCGTTAAAGAGTGTTTGTGTGC

KK_011

GTACGCGTCGACGCGCTTCCCCATCCCAAACCACC

Amplification of sseJ plus native promoter, SalI and XhoI cut sites

KK_012

GTACGCCTCGAGTTCAGTGGAATAATGATGAGCTATAAAACTTTCTAACATTATGGC

KK_013

GTACGCGTCGACGCGACGGGCGCTCACCAATC

Amplification of steA plus native promoter, SalI and XhoI cut sites

KK_014

GTACGCCTCGAGATAATTGTCCAAATAGTTATGGTAGCGAGCTTTTATGTCGG

KK_015

GTACGCGTCGACGAAGAGAACAACGGCAAGTTACAGGATCCGC

Amplification of sseABCDEFG operon promoter, SalI cut site

KK_016

GATTGTTATTTTCACGTGCCCCTCCATATACACGATAGATAATTAACGTGCTAAC

KK_017

CTATCGTGTATATGGAGGGGCACGTGAAAATAACAATCAATAGGTATGATGATGAAAG

Amplification of sscB chaperone and sseF

KK_018

GTACGCCTCGAGTGGTTCTCCCCGAGATGTATGATCAG

Reverse primer to amplify sseF and sscB, XhoI cut site

KK_019

GTACGCCTCGAGGAGTGAACGCTCCATATATTTTCTCCCAGAGACAG

Amplification and linearization of pPipB2-2HA, XhoI cut site

KK_020

CGAATTTTATAGTGAAAATATTCTCGAGTATCCGTATG

  1. Bold and underline indicate restriction endonuclease cut sites.
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