Figure 6

Evaluation for 25(OH)D₃ detection in Mice serum. (a,b) Fluorescent spectrum and fluorescent intensity of real time samples analysis using 50% mice serum spiked up samples with 25(OH)D₃ using GO Inset: Peak fluorescence change shows a linear relationship (0–1.25 µg/mL) with 25(OH)D₃ concentration. (c,d) Fluorescent spectrum and fluorescent intensity of real time samples analysis using 50% mice serum spiked up samples with 25(OH)D₃ using GO-COOH Inset: Peak fluorescence change shows a linear relationship (0–1.25 µg/mL) with 25(OH)D₃ concentration. (Apt –100 nM, GO and GO-COOH-50 µg/mL) F0: Aptamer + GO (b) and Aptamer + GO-COOH (d); and F1: Apt + GO + 25(OH)D₃ (b) and Apt + GO-COOH  + 25(OH)D₃ (d).