Figure 4
From: Lats2 promotes heart failure by stimulating p53-mediated apoptosis during pressure overload
The role of Lats2 in mediating expression of Bcl-2 family proteins during pressure overload. (A), Non-transgenic (NTG) and Lats2 +/- mice were subjected to either transverse aortic constriction (TAC) or sham operation for 4 weeks. The levels of Bcl-2, Bcl-xL, Bax, Bak, Bad and GAPDH were evaluated with immunoblot analyses. Representative images (left) and quantification (right) are shown (n = 4). *p < 0.05 versus NTG mouse hearts with sham operation. #p < 0.05 versus NTG mouse heart with TAC for 4 weeks. (B–E), Cultured neonatal rat ventricular cardiomyocytes were transduced with adenovirus harboring LacZ or Lats2. B, The levels of Bax, Bak, Lats2 and GAPDH were evaluated with immunoblot analyses. Representative images (left) and quantification (right) are shown (n = 4). (C), mRNA levels of Bax and Bak were evaluated with qPCR analyses. *p < 0.05 versus Adenovirus harboring LacZ. (D), The levels of Bax, Bak, Lats2 and α-Tubulin were evaluated with immunoblot analyses. Representative images (left) and quantification (right) are shown. (n = 3). (E), mRNA levels of Bax and Bak were evaluated with qPCR analyses. (n = 6). *p < 0.05, **p < 0.01, ***p < 0.001 versus Adenovirus harboring LacZ. (F), Cultured neonatal rat ventricular cardiomyocytes were transduced with adenovirus harboring shRNA Lats2 or shRNA control. The levels of Lats2, Bcl-2, Bcl-xL, Bax, Bak, p53 and α-tubulin were evaluated with immunoblot analyses. Representative images (left) and quantification (right) are shown (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 versus Adenovirus harboring shRNA control.
