Figure 4
From: Robust, fiducial-free drift correction for super-resolution imaging
3D drift correction applied to simulated 40 nm diameter rings separated by 80 nm. Simulated PSFs and drift curves were used. 50,000 frames were generated, divided into 100 datasets of 500 frames each. (a) True image of the original random emitters confined to the two rings. (b) Drifted image. The emitters are color-coded by frame number. (c) The drift-corrected image produced by driftCorrectKNN using default settings. (d–f) True, drifted and drift-corrected images in which multiple (10) pairs of rings centered at different x, y, z-positions were simulated. (g, i) Zoomed-in true and drift-corrected ring pair [lowest one shown in (d, f)]. (h) Corresponding estimated drift plot for the multiple ring pair example. (j, l) The differences between the estimated and simulated x, y, z-drifts as a function of the absolute frame number for (j) the single pair of rings example, (l) the multiple ring pair example. (k) Estimated versus simulated y-drift for the single pair of rings example.
