Figure 1
The evolution of migration and morphology of NIH fibroblasts over the cell cycle. (A) Green fluorescent protein (GFP)-labelled single NIH3T3 fibroblasts monitored at 3-min time intervals over an entire cell cycle beginning at cell-division. The time-evolved cell outlines and corresponding cell centroids over the entire cell cycle of a fibroblast are shown. Scale bar: 20 µm. (B) Scatter plots (left) and Box plots (right) showing the dynamic and morphological evolutions of single NIH 3T3 fibroblasts during the cell cycle progression. The cell phenotypes, including instantaneous speed, area, aspect ratio, and circularity were monitored at 3-min time intervals throughout the cell cycle. The perpendicular lines separate periods exhibiting different cell phenotypes. (C) The schematic of the time course alignments of live, single cells tracked over 20 h, related to Fig. 1D. The series of micrographs show the morphological evolution of the GFP-labeled NIH 3T3 fibroblasts over the whole cell cycle. Scale bar: 20 µm. (D) The box plots verifying cell phenotype evolutions over the cell cycle using 20 cells. (E) The plot representing the time of the optimal profiles of double-thymidine-synchronized cells that propagate to the early G1, the late G1, the S, and the G2 phase, determined as 9, 15, 2, and 5 h, respectively (and denoted as t9, t15, t2, and t5, respectively). (F) The box plots showing the dynamic and morphological measurements of synchronized NIH 3T3 fibroblasts (n > 20) in different stages of the cell cycle phases. Data information: *** indicates that the group of data is statistically significant compared to other groups. The statistical results of this figure are listed in Supplementary Table 1.
