Figure 2 | Scientific Reports

Figure 2

From: CRISPR/Cas9-mediated base-editing enables a chain reaction through sequential repair of sgRNA scaffold mutations

Figure 2

Screening of inactive sgRNAs with scaffold mutations in yeast. (A) Schematic representation of the scaffold region of sgRNAs. (B) Schematic representation of part of the scaffold region of sgRNAs with T > C mutations and PAM insertion. (C–E) Schematic representation (C) and results of the canavanine assay with nCas9-CDA (D) or Cas9 (E). Yeasts transduced with the indicated sgRNA were grown on plates with (right column) or without (left column) canavanine.

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