Figure 6
Screening of non-responsive TATAloxP with T > C mutations in mammalian cells. (A) Schematic representation of the EGFP cassette with mutated TATAloxP and polyA sequences under the EF1α promoter. (B) Experimental scheme used in (C,D). 293 T cells were transduced with the EGFP/TATAloxP cassette, and the mutant EGFP-expressing 293 T cells were subsequently transfected with Cre-R32V. (C,D) Fluorescence images of 293 T cells expressing EGFP/ various TATAloxP with the indicated T > C mutations and Cre-R32V. (E) Experimental scheme as in (F,G). 293 T cells were transfected with EGFP/TATAloxP cassette and Cre-R32V. (F,G) Fluorescence images (F) and FACS plots (G) of 293 T cells expressing EGFP/various TATAloxP with the indicated T > C mutations and Cre-R32V. WT; wild type.
