Figure 2
From: miR1908-5p regulates energy homeostasis in hepatocyte models
Cellular miR-1908-5p expression is proportional to media glucose concentration in HuH-7 cells. HuH-7 cells were maintained in variable concentrations of glucose (in pyruvate-free media) for 72 h. Cells were lysed, RNA isolated and miR-16, RNU48 and miR1908-5p were quantified by qRT-PCR. Values were normalized using the ΔCp method, using RNU48 and miR1908-5p average value as a reference. Results are expressed relative to the corresponding euglycemic (1 g/L) condition value. The error bars depict the SD. X g/L glucose vs 1 g/L glucose: **p < 0.01; *p < 0.05.
