Figure 5
From: miR1908-5p regulates energy homeostasis in hepatocyte models
STK11 is a target of miR1908-5p in HuH-7 and hepatocytes. (a) Reduced STK11 in HuH-7 and Hepatocytes treated with miR1908-5p or control miRNA for 72 h. RNA was isolated and analyzed by qPCR. Expression was internally normalized to PPIA and results are shown as a ratio of the miR1908-5p treated values divided by the miRNA control values. Control values in HuH-7 were 8.7-fold higher than the Hepatocyte values (p = 0.0014). Quantification was performed for 3 and 5 independent experiments for HuH-7 and primary hepatocytes, respectively. Error bars represent the 95% C.I. **p < 0.01; ***p < 0.001; MIM vs MIMctl). Dashed line highlights the control value of 1. (b) Reporter assay evaluating the contribution of the STK11 3′UTR, in the presence of MIM or MIMctl. HuH-7 cells were sequentially transfected with miR1908-5p (or mimic ctl) and the indicated amounts of pGL3-PromoterLKB-UTR reporter constructs, either Wt or deleted of putative miR19018-5p binding sites. Quantification of 3 biological replicates (average ± S.D), normalized for each experiment to the mimic control values is shown. Deleted samples values were not statistically significantly different between the miR1908-5p and the mimic control. *p < 0.05 between Wt and deleted constructs.
