Figure 7
From: miR1908-5p regulates energy homeostasis in hepatocyte models
Exogenous LKB1 is insufficient for AMPK phosphorylation recovery and is sensitive to miR1908-5p. HuH-7 cells (24 well plate) treated either with a miR1908 mimic or a control mimic were transfected with 20 or 100 ng of pHAGE-STK11 (or PLVX-YFP) and analyzed by western blot with the indicated antibodies. Ponceau shown represent the regions matching the AMPK (50–60 kDa) and ACC1 (250 kDa) signals. (a) representative Western blots (3 distinct gels, with matching Ponceau or TUBB signal). (b) quantification of relative phosphorylation from 2 independent determinations, normalized to the MIMIC ctl value (± S.D.); 20 ng plasmid resulted in a 12% LKB1 increase while 100 ng yielded a 3.2-fold increase (over matching YFP control). (c) recombinant LKB1 is affected by miR1908-5p. LKB1 levels were measured relative to TUBB in 4 distinct experiments performed as in (b). Bars represent the relative LKB1 abundance, expressed as the MIMIC LKB1/TUBB value over the corresponding MIMICctl value. MIM vs MIMctl: *p < 0.05; **p < 0.01.
