Figure 8
From: miR1908-5p regulates energy homeostasis in hepatocyte models
Suppression of the PI3K/AKT/mTOR axis by miR1908-5p. Western blot analyses of HuH-7 cells treated for 72 h with either Mimic inhibitor or miR1908-5p mimic, or their matching controls. (a) AKT phosphorylation. Ponceau of the 50 kDa region as loading control is shown. Representative Western blots (2 distinct blots) on the left. Right, the ratio of phospho to non-phospho signal normalized to the matching miRNA (mimic or inhibitor control) value, from 3 biological replicates (average ± S.D). (b) PDK1, ERK and MTOR phosphorylation. Representative Western blot on the left (single reprobed blot). Right, quantification of 3 biological replicates (average ± S.D), relative to the TUBB signal and normalized to the matching control oligo value. MIM or INH vs matching ctl: *p < 0.05; **p < 0.01.
