Figure 3

ACE-2 and TMPRSS-2 immunoreactivity in lung alveoli in a murine model of ageing, hormonal manipulation, and myocardial injury. The violin plots provide information on the full data distribution. The horizontal black line indicates the median of the kernel density plot. (A) ACE-2 immunoreactivity in young (left panel) and aged (right panel) control animals, gonadectomized animals, and gonadectomized animals receiving long-term hormone replacement following ischemia-reperfusion injury. (B) TMPRSS-2 immunoreactivity in young (left panel) and aged (right panel) control animals, gonadectomized animals and gonadectomized animals receiving long-term hormone replacement following ischemia-reperfusion injury. (C) Soluble angiotensin-converting enzyme (sACE-2) serum levels in young mice at baseline and following ischemia/reperfusion injury. Ordinal logistic regression was performed to test the association between the level of expression of ACE-2 and TMPRSS-2 with age, sex, and hormonal status. The resulting p-values are reported in the box. Unpaired Student’s t-test was applied for comparison of sACE-2 (only the significant p-value is reported). ACE-2, angiotensin-converting enzyme 2; Gx, gonadectomy; HR, hormone replacement; sACE-2, soluble angiotensin-converting enzyme; TMPRSS-2, transmembrane protease serine 2.