Figure 2
From: Transgenic refractory Aedes aegypti lines are resistant to multiple serotypes of dengue virus
Detection of microRNA expression levels in transgenic mosquitoes. Artificial miRNAs expressed in the midgut and carcass of female transgenic mosquitoes from (a) AeCPA-8miR and (b) AePUB-8miR lines detected through qPCR. Total RNA was isolated from mosquito midguts dissected at 0 and 16 h post blood meal (PBM). RT and qPCR were conducted as described in the “Methods” section. Data are presented as relative expression levels compared with aae-miR-100. Statistical comparisons of expression levels before and after taking a blood meal for each miRNA were made using t-tests (with adjustments for multiple comparisons). Each bar represents as mean ± SEM. Significant p values are indicated by asterisks: *p < 0.05, **p < 0.01, ***p < 0.001.
