Figure 4

DENV challenge experiments on AePUB-8miR mosquitoes at day 7. We conducted DENV challenge experiments on the transgenic Aedes mosquito line AePUB-8miR. Seven days after a blood meal containing DENV, we collected the DENV-infected mosquitoes and performed plaque assays using BHK21 cells. We performed virus challenges using (a) DENV-1 of the Myanmar strain, (b) DENV-2 of the NGC strain, (c) DENV-3 of the 98TW503 strain, and (d) DENV-4 of the H241 strain on the AePUB-8miR and control mosquitoes (Orlando strain). For each mosquito line, the mean viral titer is plotted, and the standard error of the mean is indicated. Uninfected mosquito samples were not plotted or included when determining the mean or standard error of the mean (n = 32–40). The statistical test is the Mann–Whitney rank sum test was of GraphPad Prism (version 5.0, http://www.graphpad.com) to calculate and analyze the difference in the virus titers. (e–h) The infection rates (IRs) of the mosquito lines are indicated as percentages. IR was defined as the number of virus positive midgut samples divided by the total number tested. A t-test was used to analyze the difference in the mosquito IRs. The error bars correspond to the confidence intervals (95%). All data are represented as mean ± SEM. Significant p values are indicated by asterisks: *p < 0.05, **p < 0.01, ***p < 0.001.