Figure 6
From: Transgenic refractory Aedes aegypti lines are resistant to multiple serotypes of dengue virus
Transgenic mosquitoes have reduced virus transmission potential. At 7- and 14-dpi with DENV-1 to -4, the salivary glands and the saliva were collected in female transgenic and control (Orlando strain) mosquitoes. Viral suppression was determined by plaque assay. (a,d,g,j) In salivary glands. (b,e,h,k) In saliva. Saliva samples were collected after 30 min in a P20 tip containing 5 μL of FBS and then expelled into 45 μL of L-15 media for analysis. For each mosquito line, the mean viral titer is plotted, and the standard error of the mean is indicated. Uninfected mosquito samples were not plotted or included when determining the mean or standard error of the mean. Each sample corresponds to three replicates (3 × 12 mosquitoes). The statistical test is the Mann–Whitney rank sum test was of GraphPad Prism (version 5.0, http://www.graphpad.com) to calculate and analyze the difference in the virus titers. (c,f,i,l) The transmission efficiencies of infection of the mosquito lines are indicated as percentages, which are defined as the number of positive saliva samples (i.e., successful transmission events) divided by the number of tested samples. A t-test was used to analyze potential differences in the mosquito transmission efficiency assay. The error bars correspond to the 95% confidence intervals. All data are represented as mean ± SEM. Significant p values are indicated by asterisks: *p < 0.05, **p < 0.01, ***p < 0.001.
