Figure 5 | Scientific Reports

Figure 5

From: The distinct effects of P18 overexpression on different stages of hematopoiesis involve TGF-β and NF-κB signaling

Figure 5

Overexpression of P18 from D10 promotes hematopoietic differentiation. P18/hESCs were co-cultured with AGM-S3, and P18 was induced from D0, D2, D4, D6, D8, D10, or D12. Flow cytometry at D14 with 7-AAD and the antibodies against (a) CD34/CD43, (b) CD34/CD45, (c) CD71/GPA, (d) CD34/CD43/GPA/CD41a (For EMkP) revealed that most of the aforementioned cell populations were dramatically expanded by induction of P18 from D10, especially the CD34−CD43+, CD34−CD45+, GPA+CD71+, and EMkP populations, indicating that P18 overexpression strongly promoted hematopoietic differentiation at the late stage. All results were expressed as means ± SD of three repeated experiments, and p < 0.05 was considered significant (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

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