Figure 3

Characteristics of liposomes encapsulating PSA promoter-driven perforin expression vector. (A) A schema of liposomes encapsulating PSA promoter-driven perforin expression vector (pLipo). (B) The diameter of empty Lipo and pLipo were measured using a particle size analyzer. (C) PC-3 and 22Rv1DR cells (2.0 × 104) were seeded on a 96-well plate and pLipo containing 0.5 μg of perforin expression vector were added to each well. Forty-eight h after treatment, the perforin expression level in conditioned medium was measured by ELISA. Data shown are mean with SD (n = 3 in each group).