Figure 3
From: Modulation of MagR magnetic properties via iron–sulfur cluster binding
clMagR serve as carrier protein to accept [2Fe–2S] cluster from IscU in vitro. (a) A cartoon schematically illustrates the experimental procedures of in vitro iron–sulfur cluster transfer from IscU to clMagR. (b, c) The UV–Vis absorption (b) and CD spectra (c) of IscU. IscU protein samples were taken before mixing with apo-clMagR (holo-IscU, black lines) and after incubated with apo-clMagR for 180 min (pink lines). (d, e) The UV–Vis absorption (d) and CD spectra (e) of clMagR. clMagR samples were taken before mixing with holo-IscU (apo-clMagR, light green lines) and after incubated with holo-IscU for 180 min (holo-clMagR, brown lines).
