Figure 4

B. anthracis has metabolic profile dependent on nutrient availability. (a) Comparison of ordered lists of maximum metabolic rates between nutrient-restricted (minimal media) and enriched (serum) environments. Color gradient shows rank of nutrients by their metabolic rate, with green and red representing high and low rates. The ordered list from nutrient restricted condition (left) is shown ordered, and corresponding rank from nutrient enriched condition (right) is placed side as comparison. (b) Differences of average metabolic rates between nutrient-restricted and enriched conditions by nutrient category. Bar graphs show differences between average maximum metabolic rates for nutrients by their categories (blue: carbohydrates, red: amino acids, green: lipids). Error bars represent standard error of the mean (n = 3, unpaired Student’s t-test). (c) Differences of average metabolic rates from nutrients by pathway classification. For each class of pathways, differences in maximum metabolic rates of all nutrients associated with those pathways between nutrient-restricted and enriched conditions were averaged and shown as a bar graph. Colors represent pathway classifications (blue: carbohydrates, red: amino acids, green: lipids) (*: p < 0.05, one-way ANOVA) Figure created with GraphPad Prism 5 (https://www.graphpad.com) and Tableau v2020.4.2 (https://www.tableau.com).