Figure 4 | Scientific Reports

Figure 4

From: Recurring exposure to low humidity induces transcriptional and protein level changes in the vocal folds of rabbits

Figure 4

RT-qPCR for differential gene expression. Relative quantification for each gene was determined by the 2(−ΔΔCt) method (n = 6 per humidity group except for three outlying values removed). HPRT1 was used as an endogenous control. Individual ΔΔCt was calculated for each sample using the average ΔCts from the moderate humidity group for the respective gene. Data are reported as aggregated means of 2−ΔΔCt with standardized values for the moderate humidity group. Standard errors of the mean are represented by the error bars and were calculated from individual sample values. MUC4 (p = 0.019) and SLC26A9 (p = 0.009) exhibited significantly different expression between humidity groups. SCNNA1 exhibited a substantial fold change of expression but failed to reach statistical significance (p = 0.095).

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