Figure 3

Detection of the DNA damage marker γH2AX. Chondrosarcoma cells were irradiated with 0.5, 1, 2, 4, and 6 Gy photon (X-ray) respectively proton ion particles and analysed using immunofluorescence and immunohistochemical stainings. (a) The statistical evaluation over the course of the IR dose. (b) Representative pictures of stained γH2AX foci in a single nucleus. (c) Immunohistochemical staining of γH2AX 1 h and 24 h after IR (n = 3; × 40 magnification). The significant increase of γH2AX observed after 1 h is hardly observed after 24 h.