Table 2 Summary results (2-way ANOVA) for shell mineral (TGA) and periostracum (ATR-FTIR) organic composition, and crystal orientation (XRD). These properties were measured at the growing shell edge of Argopecten purpuratus scallops.

From: Plasticity in organic composition maintains biomechanical performance in shells of juvenile scallops exposed to altered temperature and pH conditions

Analytical Technique

Shell properties

df (source, error)

Temperature (T)

pH level

T × pH

Interaction coefficient

F

p

F

p

F

p

TGA

Water content

1, 19

3.78

0.070

11.26

0.004

0.68

0.442

 

Organic matter Phase-1

1, 19

5.19

0.037

4.08

0.060

6.38

0.022

− 0.0019

Organic Matter Phase-2

1, 19

2.14

0.163

1.90

0.187

0.14

0.709

 

CO2 Loss

1, 19

0.88

0.363

7.44

0.015

0.00

0.975

 

Total Organic Matter

1, 19

9.80

0.006

1.34

0.264

7.11

0.017

− 0.0016

ATR–FTIR

Sulfates

1, 19

1.53

0.235

7.89

0.012

3.23

0.091

 

Polysaccharides

1, 19

9.51

0.007

12.88

0.002

7.09

0.017

 + 0.0019

Proteins (amides)

1, 19

0.46

0.508

4.27

0.055

3.29

0.089

 

Carbonates (CO3)

1, 19

6.63

0.020

9.40

0.007

4.32

0.054

 

Lipids

1, 19

0.17

0.686

0.18

0.679

0.02

0.896

 

OH + Amides A

1, 19

1.70

0.210

0.14

0.711

0.27

0.611

 

XRD

Crystal Orientation

1, 19

0.27

0.611

1.24

0.282

5.37

0.035

− 0.0270

  1. F and p are F-ratio and p-values, respectively. Significant p-values (p < 0.05) are shown in bold. The coefficient identifies antagonistic (−) and synergistic (+) effects in the interaction of both environmental variables.
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