Figure 2

Flies mounted for longer experience depletion of glucose and glycogen storages. (A) Average glycogen (mg/dL) for free walking flies after 1 h (n = 10, N = 50) and 5 h (n = 8, N = 40) since anesthetization, and for mounted flies after 1 h (n = 10, N = 50) and 5 h (n = 10, N = 50) since mounting. (B) Average glucose (mg/dL) for free walking flies after 1 h (n = 10, N = 50) and 5 h (n = 8, N = 40) since anesthetization, and for mounted flies after 1 h (n = 10, N = 50) and 5 h (n = 10, N = 50) since mounting. (C) Average triglyceride (mg/dL) for free walking flies after 1 h (n = 21, N = 105) and 5 h (n = 21, N = 105) since anesthetization, and for mounted flies after 1 h (n = 21, N = 105) and 5 h (n = 21, N = 105) since mounting [*P < 0.05, **P < 0.01, ****P < 0.0001, ordinary one-way ANOVAs followed by Tukey’s multiple comparisons tests].