Figure 3
Mounted flies aerobically exert themselves more than free walking flies. (A) The activity of free walking and mounted flies were manually scored over 5 min. (B) Fraction of time spent active for free and mounted (n = 20, per condition) flies over 5 min, measured midday (C) Open-flow respirometry setup with live video recording. (D) Average CO2 production over a 10-min interval in free walking (n = 4, N = 40) and mounted flies (n = 4, N = 40), compared to machine baseline (n = 5), and FlyNap anesthetized flies (n = 4, N = 40). (E) Average CO2 production in free walking (n = 4, N = 40) and mounted flies (n = 4, N = 40) normalized to average CO2 production in FlyNap anesthetized flies (n = 4, N = 40) (F) Sample of data for continuous CO2 production in a single fly with overlayed bars representing activity bouts (n = 1). (G) Average CO2 production in single flies during periods of inactivity and during bouts of activity (N = 4). [****P < 0.0001 unpaired t tests].
