Figure 5
Fasting and restraint additively leads to excessive storage depletion and consequently to elevated feeding motivation. Removal of flies from food leads to fasting that, over time, uses energy storage which can be assessed by measuring a feeding motivation response. Acute physical restraint leads to extended bouts of activity during which we observe elevated levels of energetically demanding behaviors (leg pushing and abdomen curling). This activity depletes internal energy storage far more rapidly than fasting alone, which in turn elevates the internal hunger state and motivational feeding response at a comparatively faster rate. Consequently, the combination of fasting and restraint produces a rise in motivational feeding response over time that is ten times more rapid than from fasting alone.
