Figure 4
Microinjection of L-733,060 into the medial septum (MS) does not affect carbachol-induced suppression of CA1 PS in anaesthetized animals. (a) Composite of the microinjection sites in the MS was built as in Fig. 1. Carbachol (0.156 µg/µl, 0.5 µl) was microinjected three times (C1, C2 and C3), at least 1 h apart, with vehicle or L-733,060 (1 ×, 0.0176 µg/µl, 0.5 µl) microinjected 15 min before C2. (b, d) Representative PS traces following repeat carbachol microinjection into the MS. (c, e) Time course of change in amplitude of CA1 PS on repeat microinjection of carbachol. Note that carbachol elicited robust PS amplitude suppression which was unaffected by pre-treatment with vehicle or L-733,060. Data are presented are mean ± S.E.M.
