Figure 6
Intraseptal L-733,060 decreased nociceptive behaviours in the formalin test. In the behaving animal, formalin (1.25%, 0.1 ml) was injected into the right hind paw 15 min after microinjection of vehicle or L-733,060 into the medial (MS) or lateral (LS) septum (1 ×, 0.0176 µg/µl, MS, n = 9; LS, n = 14; 10 ×, 0.176 µg/µl, MS, n = 7). (a) Composite diagram of microinjection sites in the MS and LS built as in Fig. 1. (b, d) Time course graphs illustrating the biphasic (Phase 1, 1–5 min; Phase 2, 11–60 min) flinching and licking responses induced on injection of formalin. The behavioural response was binned in blocks of 5 min. (c, e) Histograms illustrating the cumulative number of flinches and cumulative duration of licking in Phases 1 and 2. Pre-treatment with L-733,060 in the MS and LS significantly attenuated formalin-induced flinching in phases 1 and 2 (c). However, the drug did not significantly affect licking (e). Data are mean ± S.E.M. Significant difference (p < 0.05): (b, d) *‘1 ×/10 × L-733,060 MS’ vs. ‘Vehicle MS/LS’ group, ^‘1 × L-733,060 LS’ groups vs. ‘Vehicle MS/LS’ group; two-way RM ANOVA followed by Bonferroni post-hoc test; (c) *vs. ‘Vehicle MS/LS’ group; one-way ANOVA followed by Newman–Keuls post-hoc test.
