Figure 1

Diet and NNMTi treatment status substantially altered body weight and fat mass. a After weaning, mice were placed on a high-fat diet (HFD, 60% fat) for 12 weeks, transitioned to Western diet (WD, 45% fat) for 4 weeks, and then randomized to WD or to lean diet (LD, 10% fat) and vehicle (-V) or NNMTi treatment (-T) with 5-amino-1-methylquinolinium (5A-1MQ) for approximately 7 weeks prior to study termination and cecal sample collection; a control group remained on LD throughout the study. b,c Body weight and fat mass were significantly different between the diet-induced obesity model and the lean control group at study start (22.5 weeks of age); at the end of the study, body weight and fat mass were lower in the WD/LD-V group than the WD/WD-V control group, and in the NNMTi-treated group these measures were even lower and statistically indistinguishable from the LD/LD-V control group when a correction for multiple comparisons was not run (n = 6–8). Graphs depict mean + /− SEM; unless specified, multiple comparisons were significant before and after FDR correction (p < 0.05 and q < 0.05); further statistical details can be found in Supplementary Table S2. *, significantly different from all other groups for that study day; a, significantly different from the lean control group (LD/LD-V); b, significantly different from the obese control group (WD/WD-V); c, significantly different from vehicle-treated mice switched from WD to LD (WD/LD-V); d, significantly different from 5A-1MQ-treated mice switched from WD to LD (WD/LD-T); ^v, p > 0.05 but q < 0.05.