Figure 3
From: Reduced calorie diet combined with NNMT inhibition establishes a distinct microbiome in DIO mice
Microbial phyla exhibit dramatic shifts predominantly mediated by diet switch as opposed to diet composition. (a) Relative abundance for each of the phyla identified in the mouse cecal samples as well as the remaining sequences which could not be assigned (unclassified). (b–d) Three bacterial phyla demonstrated significant differences following Benjamini–Hochberg correction for multiple comparisons: Firmicutes, Proteobacteria, and Verrucomicrobia. (e–f) Actinobacteria and Bacteroidetes both demonstrated significant differences before (but not after) Benjamini–Hochberg correction. (g) The ratio of Firmicutes:Bacteroidetes was significantly lower in the WD/LD-T mice compared to those maintained on WD, and there was a trend for a similar effect in the WD/LD-V mice. Graphs depict mean + /− SEM; unless specified, multiple comparisons were significant before and after FDR correction (p < 0.05 and q < 0.05); further statistical details can be found in Supplementary Table S3. a, significantly different than the lean control group (LD/LD-V); b, significantly different than the obese control group (WD/WD-V); ^, p < 0.05 but q > 0.05.
