Figure 6

Fibrosis and hepatic stellate cell (HSC) activation are exacerbated in neutrophil depleted mice during inflammation resolution. Neutrophil depletion was achieved via administration of anti-Ly6G antibodies during the 2-week diet reversal-induced NASH inflammation resolution. Ly6G⁺ neutrophils (a), fibrosis (b) and α-SMA⁺ activated HSCs (c) were detected through paraffin-embedded tissue sections stainings and analyzed by ANOVA comparison between mice undergoing diet reversal alone (FFC-CD), and diet reversal in association with either IgG (FFC-DC + IgG) or anti-Ly6G antibodies (FFC-CD + anti-Ly6G) administration. mRNA expression of genes associated with inflammation resolution and HSC activation was assessed comparing fold-changes between the mentioned groups. Hydroxyproline levels were also compared among the mentioned groups by ANOVA followed by the Turkey post hoc test (d,e).