Figure 7
Phenotypic switch into pro-resolution macrophage population and release of pro-repairing mediators are impaired in neutrophil depleted mice during inflammation resolution. Detection of CD163+ pro-restorative macrophages (a), pro-inflammatory Ly6Chi monocytes/macrophages (b) and infiltrating CD11b+ myeloid cells (c) were detected via IHC and quantified for ANOVA comparison between the groups receiving diet reversal alone (FFC-CD), and diet reversal in association with either IgG (FFC-CD + IgG) or anti-Ly6G (FFC-CD + anti-Ly6G). mRNA expression was compared and expressed as fold changes. Monocyte chemoattractant receptor ccr2 (d) and changes in the expression of pro- (mmp2, timp1) versus anti-fibrotic (mmp8, mmp9, mmp10) mediators were compared among the mentioned groups (e). (f) Western blots for protein expression assessment was performed targeting pro-restorative macrophage marker arginase 2 (cropped from Supplementary Fig. 4) and pro-fibrotic MMP-2 (cropped from Supplementary Fig. 5b). α-tubulin expression (band imported from Supplementary Fig. 4) was illustrated as housekeeping control.
