Figure 4

(A,B) Microscopic images (fluorescence and bright field) of MCF-7 and MCF-10 A cells, incubated with CND (5% vol./vol.) for 30 min. Quantification of fluorescence of cellular CND, in this experiment, is shown in (B). (C,D) Microscopic images (fluorescence and bright field) of MCF-7 and MCF-10 A cells, incubated with CND (5% vol./vol.) for 30 min and kept under different temperature (37 °C and 42 °C) for another 30 min. Quantification of fluorescence of cellular CND, in this experiment, is shown in (D). (E,F) Microscopic images (fluorescence and bright field) of MCF-7 and MCF10-A cells, incubated with CND (5% vol./vol.) for 30 min and kept under different pH (6.0–7.5) Quantification of fluorescence of cellular CND, in this experiment, is shown in (F). (AFU stands for arbitrary fluorescence unit).