Figure 3
From: High-throughput formation and image-based analysis of basal-in mammary organoids in 384-well plates
Screening the effects of Matrigel and growth factor concentration on organoid formation. Summary of organoid formation under 63 different Matrigel and growth factor combinations at (a) day 2 and (b) day 16. Projected area, circularity, and whether the structure is aggregated or not aggregated are represented by the marker size, color, and shape respectively. Each marker represents a different replicate in a quadruplicate study. Conditions are divided by growth factor type (HB-EGF: left, EGF: right), and the no growth factor conditions are duplicated on both sides for ease of visualization. (c) Statistical analyses of the most consistent conditions with 4 out of 4 wells aggregated at day 16 are shown. Conditions not connected by the same letter have significantly different areas (ANOVA and post-hoc Tukey test: P < 0.05). Conditions depicted in Fig. 2 are labeled.
