Figure 2 | Scientific Reports

Figure 2

From: In silico analysis of local RNA secondary structure in influenza virus A, B and C finds evidence of widespread ordered stability but little evidence of significant covariation

Figure 2

(Left) ScanFold data for IAV 7 ( +), with motif 7_1 and 7_4 designated by a blue box. M1 and M2 versions of this transcript are illustrated for reference. The base pair track (bp track) shows arcs correlating to base pairings, where blue arcs have a <  −2 score, green arcs have a < −1 z-score, yellow arcs have a < 0 z-score, and gray arcs have a z-score > 0. (Right) Motif 7_1 correlates with the published multibranch loop23, aligning with our reference sequence after global refold (gray boxes). Motif 7_4 has an extended hairpin that occludes any formation of the 5′ pairing or pseudoknot structure around the 3′ splice site (739–740, purple triangles), but a global refold reveals the published 3′ pairing25. Two sequence variations from our reference sequence are annotated at 136 and 655. ScanFold z-scores are overlaid in each nucleotide circle, with blue designating < −2 z-score. Structural images were adapted from VARNA, and the genome illustrations were adapted from NCBI.

Back to article page