Figure 3

ScanFold analysis for motifs (top to bottom panels, respectively) IAV ( +) 4_1, IAV (−) 6_3, IBV (−) 1_3, and IBV (−) 4_2 (locations designated with blue boxes). The base pair track (bp track) shows arcs correlating to base pairings, where blue arcs have a < −2 score, green arcs have a < −1 z-score, yellow arcs have a < 0 z-score, and gray arcs have a z-score > 0. The R-Scape calculations showed observed base pair covariance (highlighted in green), but the number of observe covarying pairs fell below the expected value (given the sequence alignment). ScanFold per nt zavg are overlaid in each nucleotide circle, with blue designating < −2 zavg. Structural images were adapted from VARNA, and the genome illustrations were adapted from NCBI.