Figure 3 | Scientific Reports

Figure 3

From: Combined effects of double mutations on catalytic activity and structural stability contribute to clinical manifestations of glucose-6-phosphate dehydrogenase deficiency

Figure 3

Fluorescence emission spectra of recombinant human G6PD variants. (A) Intrinsic fluorescence spectra. The emission spectra of G6PD proteins were recorded over the range of 300–400 nm using an excitation wavelength of 295 nm. (B) ANS fluorescence spectra. G6PD proteins were incubated with 100 μM ANS at 25 °C for 1 h and emission spectra were monitored between 400 and 600 nm using an excitation wavelength of 395 nm. The vertical dash-line represents the fluorescence emission maximum of the WT enzyme. Insets: magnified view of the fluorescence emission.

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