Figure 4 | Scientific Reports

Figure 4

From: Combined effects of double mutations on catalytic activity and structural stability contribute to clinical manifestations of glucose-6-phosphate dehydrogenase deficiency

Figure 4

Thermal stability analysis of recombinant G6PD variants. G6PD proteins were heated (20 to 80 °C) in the presence of 5 × SYPRO Orange reporter dye and various concentrations of NADP+ (0, 10 and 100 μM) or AG1 (10 and 100 μM). Protein unfolding was monitored by measuring the emission of the fluorescence dye at 580 nm. Experiments were performed in triplicate, and the data were averaged and plotted before calculating the Tm using GraphPad Prism.

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