Figure 2 | Scientific Reports

Figure 2

From: Human IgE does not bind to human FcRn

Figure 2

Titration of human sFcRn in SPR reveals no binding to IgE at pH 7.4 or 6.0. Anti-biotin IgG variants and IgE were captured on a sensor with different concentrations of BSA-biotin spotted, leading to comparable levels of antigen-bound IgG and IgE (not shown due to blank subtraction). Human sFcRn was injected in a twofold serial dilution covering a concentration range from 15.63 to 1000 nM and KD values were calculated fitting a 1:1 Langmuir binding model after blank subtraction. A representative of three independent experiments is shown.

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