Figure 5

Capturing autophagic flux increase through serum starvation and HBSS starvation. (A) HeLa cells were kept in standard medium (10% FBS) or in FBS-reduced medium (1.5%) for 4 or 24 h. Medium change was combined with (co)-treatment of cells with 2.5 nM BafA and/or 1.5 or 12.5 µM rapamycin. Shown are representative Western blots of LC3 and actin (top) as well as quantifications (n = 3; mean ± SEM; bottom). Statistics: One-sample t-test, compared to control (2.5 nM BafA). (B) HeLa cells were kept in standard cultivation medium or HBSS for 3 h in the presence or absence of 2.5 nM BafA and/or 1.5 µM or 12.5 µM rapamycin. Shown is a representative Western blot for LC3, p62/SQSTM1 and actin (left) and quantification of Western blot data from two independent experiments (right).