Table 1 Zika virus CD8 peptide selection based on multiparameters using immunoinformatics tools.

From: In silico construction of a multiepitope Zika virus vaccine using immunoinformatics tools

Protein

Epitope0

Size (aa)

Position

Nº of alleles1

% of total alleles2

Immunogenicity score (IEDB)3

Nº of HLAs w/ Percentile Rank ≤ 204

Nº of HLAs with SMM IC50 ≤ 500 nM5

Nº of HLAs with ANN IC50 ≤ 500 nM6

Capsid Protein

VSPFGGLKRLP (C_24-34)

11

24–34

17

42.5

0.0036

12

1

1

prM Protein

IFRNPGFALAAAAIA (prM_129-143)

15

129–143

16

34.04

0.3976

49

4

8

Envelope Protein E

SQEGAVHTALA (E_260-270)

11

260–270

10

25

0.23948

15

3

3

Envelope Protein E

VRGAKRMAVLG (E_415-425)

11

415–425

10

25

− 0.21504

15

2

1

Envelope Protein E

MQTLTPVGRLI (E_349-359)

11

349–359

9

22.5

0.13774

26

3

2

NS1

WRSVEGELNAILEENGVQL (NS1_68-86)

19

68–86

11

23.40

0.5434

34

3

3

NS2A

ALIAAFKVR (NS2A_78-86)

9

78–86

5

10.64

0.07299

8

1

1

NS2B

SEVLTAVGLICAL (NS2B_5-17)

13

5–17

22

46.81

0.24318

40

8

7

NS3

VREAIKTRLRTVIL (NS3_209-222)

14

209–222

14

29.79

0.26841

34

6

6

NS4A

GKMGFGMVTLG (NS4A_79-89)

11

79–89

9

22.5

0.01824

24

4

4

NS4A

GAAFGVMEALGT (NS4A_1-12)

12

1–12

12

30

0.17747

29

4

3

NS4B

TPLTLIVAI (NS4B_105-113)

9

105–113

13

27.66

0.20764

10

2

1

NS5

YMWLGARFLEFEAL (NS5_477-490)

14

477–490

17

36.17

0.6005

54

6

6

  1. 0—Underlined are the first aminoacids of a 9-mer peptide predicted as having percentile rank ≤ 20 as calculated by IEDB (http://tools.iedb.org/mhci/).
  2. 1—Total number of HLA-I covered by the peptide as predicted by Propred1. All 47 alleles were selected, and the percentage of the highest scoring peptides, the Immuneproteasome and the Proteasome filter threshold was set up as 3%.
  3. 2—Percentage of HLA-I molecules identified as binders in relation to the total number of HLA-I available.
  4. 3—Immunogenicity score obtained with http://tools.iedb.org/immunogenicity/. Positive values are considered immunogenic.
  5. 4—Number of HLA that presented percentile rank lower than 20 as predicted by IEDB (http://tools.iedb.org/mhci/).
  6. 5—Number of HLAs with SMM IC50 ≤ 500 nM as predicted by IEDB (http://tools.iedb.org/mhci/).
  7. 6—Number of HLAs with ANN IC50 ≤ 500 nM as predicted by IEDB (http://tools.iedb.org/mhci/).
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