Table 2 Zika virus CD4 peptide selection based on multiparameters using immunoinformatics tools.

From: In silico construction of a multiepitope Zika virus vaccine using immunoinformatics tools

Protein

Epitope

Size (aa)

Position

Nº of alleles1

% of total alleles2

Immunogenicity (Percentile Rank)3

Nº of HLAs w/Percentile Rank < 204

Nº of HLAs w/ SMM align IC50 < 500 nM5

Nº of HLAs w/NN align IC50 < 500nM6

IFNepitope Score (SVM)7

Capsid Protein

HGPIRMVLAILAFLR (C_41-55)

15

41–55

37

72.54

24.3268

21

17

14

0.4521

prM Protein

TTSTWVVYGTCHHKK (prM_71-85)

15

71–85

31

60.8

65.45964

4

0

2

− 0.1269

Envelope Protein E

EYRIMLSVHGSQHSG (E_136-150)

15

136–150

33

64.7

40.45516

13

9

12

− 0.1841

Envelope Protein E

DKLRLKGVSYSLCTA (E_296-310)

15

296–310

34

66.6

46.33096

12

2

12

− 0.3609

NS1

SYFVRAAKTNNSFVV (NS1_121-135)

15

121–135

30

58.8

31.18672

11

12

12

0.3363

NS2A

PFVMALGLTAVRLVD (NS2A_196-210)

15

196–210

29

56.8

42.90484

17

11

14

0.2505

NS2B

FAAGAWYVYVKTGKR (NS2B_116-130)

15

116–130

11

27.50

42.51056

14

5

9

0.5012

NS3

LRGLPVRYMTTAVNV (NS3_236-250)

15

236–250

35

68.6

43.8254

12

8

13

0.4513

NS4A

LGIFFVLMRNKGIGK (NS4A_66-80)

15

66–80

40

78.4

25.47468

21

10

14

0.0979

NS4B

HYMYLIPGLQAAAAR (NS4B_119-133)

15

119–133

18

35.29

42.61168

19

10

14

0.0579

NS4B

VAVSSAILSRTAWGW (NS4B_178-192)

15

178–192

38

74.5

49.2798

13

6

6

0.3106

NS5

LLYFHRRDLRLMANA (NS5_766-780)

15

766–780

36

70.6

38.39304

20

8

12

0.2457

  1. 1—Total number of HLA-II covered by the peptide as predicted by Propred MHC-II Binding Peptide Predicition Server. All 51 alleles were selected. The threshold was set up as 1%.
  2. 2—Percentage of HLA-II molecules identified as binders in relation to the total number of HLA-II available.
  3. 3—Immunogenicity percentile rank obtained with http://tools.iedb.org/CD4episcore/. Lower numbers are considered immunogenic (lower than 66).
  4. 4—Number of HLA that presented percentile rank lower than 20 (high affinity).
  5. 5—Number of HLA that presented SMM IC50 lower than 500 nM (high affinity).
  6. 6—Number of HLA that presented NN IC50 lower than 500 nM (high affinity).
  7. 7—IFN-γ induction score. Positive values indicate IFN-γ induction.
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